|
KMID : 0364219830260030171
|
|
Korean Journal of Zoology
1983 Volume.26 No. 3 p.171 ~ p.179
|
|
|
Effects of 3-Aminobenzamide on DNA Strand Breaks and Excision Repair in CHO cells Exposed to Methyl Methanesulfonate and Ultraviolet-light
|
|
Park Sang-Dai
Jang Young-Ju
Roh Jung-Koo
|
|
|
Abstract
|
|
|
|
Amounts of DNA single strand breaks and unscheduled DNA synthesis in CHO cells exposed to MMS were increased in the presence of 3-aminobenzamide, a potent inhibitor of poly (ADP-ribose) polymerase. However, those in cells irradiated with UV-light were decreased. These results suggest that poly (ADP-ribose) polymerase acts negatively on the MMS-induced base excision repair but positively on the UV-induced nucleotide excision repair. In the combined treatment with MMS and UV-light in the presence of this inhibitor, amounts of strand breaks were just the same as those in the absence of the inhibitor. But those of unscheduled DNA synthesis were increased up to the amount induced by UV-light alone. These results may suggest that poly (ADP-ribose) polymerase affects the incision step of excision repair induced by MMS and UV-light independently, and that it may potentiate the complete cleaving of UV-induced pyrimidine dimers possibly by the repair enzymes which might have been partially inactivated by MMS.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|